Deoxyribozyme-Catalyzed Labeling of RNA

摘要

Site-specific covalent modification of RNA is important for enabling structure-function studies. For example, probes such as fluorescein are commonly used in fluorescence resonant energy transfer (FRET) investigations of RNA folding. Biotin is used for immobilization during single-molecule analysis, to enable RNA-protein cross-linking studies, and as a key element of selection schemes in vitro. The 5' and 3' termini of RNA may be derivatized, but many experiments instead demand internal modification, and no direct methods are known for site-specific modification within an arbitrary RNA sequence.