Infectivity Analysis of the Cloned Component of a Monopartite Begomovirus-Betasatellite Complex Causing Tomato Leaf Curl Disease in Pakistan

摘要

Cotton leaf curl Rajasthan virus (CLCuRaV), a monopartite begomovirus causing devastating losses of cotton crop in India, was isolated from tomato (Solatium lycopersicum) along with DNA satellite (CLCuRaB~(tomato)) and characterized in Faisalabad, Pakistan. Infectious clones of CLCuRaV~(tomato) and CLCuRaB~(tomato) were produced and transformed into Agrobacterium strain GV3101. A partial tandem repeat construct of CLCuRaV~(tomato) was inoculated to Nicotiana benthamiana and tomato plants alone and/or with CLCuRaB~(tomato). CLCuRaV~(tomato) alone produced very mild symptoms in N. benthamiana plants, whereas tomato plants showed no symptoms by inoculation with CLCuRaV~(tomato) in the absence of CLCuRaB~(tomato). In N. benthamiana plants 100% infection followed by severe symptoms was observed while in tomato plants 70-80% infection with mild symptoms was observed on co-inoculation with CLCuRaV~(tomato) and CLCuRaB~(tomato). Symptoms severity of CLCuRaV~(tomato)~ increased in the presence of CLCuRaB~(tomato) in N. benthamiana and tomato plants. Here we demonstrated the first experimental pathogenicity analysis of a cotton virus isolated from tomato plants both in N. benthamiana and tomato plants.