Inhibitory effects of microalgae on the activation of hyaluronidase

摘要

The inhibitory effects of seven microalgae, Nostoc flagelliforme, Spirulina platensis, Porphyridium purpureum, Rhodosorus marinus, Chlorella pyrenoidosa, Dunaliella salina and Pleurochrysis carterae on the activation of hyaluronidase were evaluated. The inhibitory effect of the ethanol-insoluble fraction of each water extract from microalgae was stronger than that of the ethanol-soluble fraction. The 50% inhibitory concentration (IC50) of the ethanol-insoluble fraction of S. platensis, P. purpureum, R. marinus, C. pyrenoidosa, D. salina and P. carterae was 0.15, 0.18, 0.26, 0.94, 0.15 and 0.41 mg mL(-1), respectively. The IC50 of N .flagelliforme was not calculated, because there was no detectable inhibitory effect of this alga. The IC50 of disodium cromoglycate (DSCG) used as the anti-allergic medicine was 0.14 mg mL(-1). The IC50 of S. platensis, P. purpureum and D. salina were almost the same as that of DSCG. This suggests that the ethanol-insoluble fraction of S. platensis, P. purpureum and D. salina might be an anti-allergic substance. The ethanol-insoluble fraction of S. platensis and D. salina was ultrafiltered through a membrane having a molecular exclusion limit of 20 kDa. The IC50 of the residue was stronger than that of the filtrate. These results suggest that the anti-allergic substance(s) of these microalgae may be polysaccharides.