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首页> 外文期刊>Journal of Animal and Feed Sciences >A highly efficient method for determination of allantoin in ruminal digesta, ovine urine and blood serum by HPLC.
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A highly efficient method for determination of allantoin in ruminal digesta, ovine urine and blood serum by HPLC.

机译:高效液相色谱法测定瘤胃消化液,绵羊尿液和血清中尿囊素的高效方法。

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摘要

A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep was described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using 3 long Nova Pac C18-columns (Waters) and UV detection at 205 and 215 nm. The low coefficient of variation and satisfactory recovery of allantoin added to assayed samples (~100%) provided a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.
机译:描述了一种直接测定绵羊瘤胃消化,尿液和血清尿囊素的高效液相色谱方法。用去离子水按1:3稀释血清和尿液样品。使用3个长的Nova Pac C18色谱柱(Waters)并在205和215 nm处进行UV检测,实现尿囊素的分离和定量。添加到被测样品中的尿囊素变异系数低且回收率令人满意(〜100%),为常规定量检测大量尿液,瘤胃消化液和血清样品中尿囊素提供了一种合适的工具。

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