DNA Markers Based on PSY Genes Can Differentiate Yellow- and White-Fleshed Loquats

摘要

White-flesh is one of the most appreciated traits in loquat. The development of new white-fleshed cultivars is costly and time consuming, involving the generation and selection of thousands of progeny each year. Marker-assisted selection (MAS) is an excellent technique to accelerate conventional breeding approaches. In this study, a specific DNA marker based on the sequence of phytoene synthase (PSY) gene was identified as an ideal DNA marker for the identification of fruit flesh color. We screened 31 cultivars derived from different countries and 24 Fj hybrids developed from 4 cross combinations, and analyzed the relationships between flesh color and the DNA marker. The results showed that the DNA marker not only can distinguish yellow- and white-fleshed cultivars accurately, but can be used for accurate and early selection of yellow and white flesh progeny derived from controlled hybridizations.