Overexpression and elevated plasma level of tumor-associated antigen 90K/Mac-2 binding protein in colorectal carcinoma

摘要

The cancer cell secretome may contain potentially useful biomarkers. Previously, we have analyzed the colorectal carcinoma (CRC) cell secretome. In this study, tumor-associated antigen 90K (TAA90K)/Mac-2 binding protein (Mac-2BP), one of the CRC cell secreted proteins, was chosen for evaluation as a potential CRC biomarker because its mRNA level was also found to be significantly elevated in CRC tissues and in a more metastatic CRC cell line from the analysis of two public domain array-based datasets. Immunohistochemical analysis of 241 CRC specimens showed that TAA90K/Mac-2BP was positively detected in 52.7% of the tumors, but weakly or not detected in over 95% of the adjacent nontumor epithelial cells. The plasma TAA90K/Mac-2BP levels were significantly higher in CRC patients (N = 280) versus healthy controls (N = 147) (7.77 6 3.49 vs. 5.72 6 2.67 mg/mL, p,0.001). Moreover, combination of TAA90K/Mac-2BP and carcinoembryonic antigen (CEA) could outperform CEA alone in discriminating CRC patients from healthy persons in this case-control study. Our results collectively indicate that analysis of cancer cell secretome is a feasible strategy for identifying cancer biomarker candidates, and the TAA90K/Mac-2BP may be a potential CRC biomarker.