首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Transfer region of a Bacteroides conjugative transposon contains regulatory as well as structural genes.
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Transfer region of a Bacteroides conjugative transposon contains regulatory as well as structural genes.

机译:拟杆菌结合转座子的转移区包含调节基因和结构基因。

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摘要

Conjugative transposons (CTns) are integrated elements that excise themselves from the chromosome to form a circular transfer intermediate that is transferred by conjugation to a recipient. In an earlier paper, the excision step was shown to be regulated by tetracycline and to be dependent on the regulatory gene, rteC. In this paper, we report that genes involved in conjugal transfer are also regulated by tetracycline but that regulation is more complex. Genes contained within a 20-kbp region that is sufficient for conjugal transfer were disrupted by single crossover integration events. Most of the disruptions abolished transfer of the CTn. None of them abolished excision. Antibodies to two of the proteins encoded in this region (TraG and TraN) were obtained and used to show that production of these proteins was dependent on tetracycline stimulation. Both TraG and TraN were membrane proteins. A surprising finding was that a disruption in the gene traQ increased transfer of CTnERL over 100-fold. Thus, TraQ may be a repressor protein that controls expression of transfer genes. If so, TraQ is not the only protein that controls expression of transfer genes because production of TraG and TraN in the traQ disruption mutant was still dependent on tetracycline stimulation.
机译:共轭转座子(CTns)是整合元件,可将自身从染色体上切除,形成环状转移中间体,通过结合转移至受体。在较早的论文中,切除步骤显示受四环素调节,并依赖于调节基因rteC。在本文中,我们报道了参与夫妻转移的基因也受到四环素的调控,但调控更为复杂。单交换整合事件破坏了20kbp区域内足以进行夫妻转移的基因。大多数中断都取消了CTn的传输。他们都没有废除切除术。获得了针对该区域编码的两种蛋白质的抗体(TraG和TraN),并用于证明这些蛋白质的产生取决于四环素的刺激。 TraG和TraN都是膜蛋白。令人惊讶的发现是基因traQ的破坏使CTnERL的转移增加了100倍以上。因此,TraQ可能是控制转移基因表达的阻遏蛋白。如果是这样,TraQ并不是控制转移基因表达的唯一蛋白质,因为在traQ破坏突变体中TraG和TraN的产生仍然取决于四环素的刺激。

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