首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Negative transcriptional regulation of virulence and oncogenes of the Ti plasmid by Ros bearing a conserved C(2)H(2)-zinc finger motif.
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Negative transcriptional regulation of virulence and oncogenes of the Ti plasmid by Ros bearing a conserved C(2)H(2)-zinc finger motif.

机译:负毒性的Ti质粒的毒力和致癌基因的负转录调控,带有保守的C(2)H(2)-锌指基序的Ros。

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摘要

The chromosomal ros gene in Agrobacterium tumefaciens encodes a repressor of virulence and oncogenes that are located on a resident Ti plasmid. Mutational inactivation of ros de-represses the expression of the virC and virD operons, causing premature processing and accumulation of T-DNA molecules, and the premature expression of the oncogene, ipt, leading to the synthesis of cytokinin in the bacterium rather than in the plant host cell. Ros is a 15.5kDa protein containing a novel "eukaryotic" C(2)H(2) zinc finger. Amino acid substitutions in the finger result in the loss of binding of Ros to the ros box, a 40bp sequence within the operator of virC/D and ipt gene promoters; and the loss of binding of a zinc ion. The ros gene is highly conserved in members of the Rhizobiaceae. Evolutionary distance tree analyses revealed distant ties to the Japanese puffer fish, Fugu rupripes rather than to plants. Interestingly, ros homologues were found in microorganisms derived from marine sources, supporting the hypothesis that ros may have originated from a marine rather than a terrestrial organism.
机译:根癌农杆菌中的染色体ros基因编码位于驻留Ti质粒上的毒力和癌基因的阻遏物。 ros的突变失活会抑制virC和virD操纵子的表达,从而导致T-DNA分子的过早加工和积累,致癌基因ipt的过早表达,导致细菌中而不是细菌中细胞分裂素的合成。植物宿主细胞。 Ros是一种15.5kDa的蛋白质,其中包含新颖的“真核” C(2)H(2)锌指。手指中的氨基酸取代导致ros与ros box的结合丢失,而ros box是virC / D和ipt基因启动子的40bp序列。以及锌离子结合力的丧失。 ros基因在根瘤菌科中是高度保守的。进化距离树分析显示与日本河豚Fugu rupripes而不是植物的远缘联系。有趣的是,在海洋来源的微生物中发现了罗斯同系物,这支持了罗斯可能起源于海洋而不是陆地生物的假说。

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