首页> 外文期刊>Polyhedron: The International Journal for Inorganic and Organometallic Chemistry >Studies of the interaction between bis(dithiocarbamato)copper(II) complexes with nitric oxide in aqueous solution and biological applications
【24h】

Studies of the interaction between bis(dithiocarbamato)copper(II) complexes with nitric oxide in aqueous solution and biological applications

机译:双(二硫代氨基甲酸酯)铜(II)配合物与一氧化氮在水溶液中的相互作用及生物应用研究

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Three new bis(dithiocarbamato)copper(II) complexes have been prepared and characterized by elemental analysis, UV-Vis, and IR spectroscopy: bis(4-piperidonedithiocarbamato)copper(II), [Cu(PdtC)(2)](2) (1), bis(piperidinedithiocarbamato)copper(II), [Cu(PpdtC)(2)](2) (2), and bis[(2-piperidinecarboxy)dithiocarbamato]copper(II), Cu(Ppidtc)(2) (3). The crystal structures of the complexes I and 2 have been determined using X-ray diffraction methods and they are dimeric. The coordination sphere of copper(II) ions is described as a distorted square-pyramid: the axial Cu-S bond distances 2.7 and 2.8 angstrom for 1 and 2, respectively, are longer than the equatorial Cu-S ones (2.3 angstrom). Complexes I and 2 are not soluble in water. In contrast, 3 is water soluble. The interaction of 31 the previously prepared bis[N-(dithiocarboxy)sarcosine]copper(II), Cu(Sdtc)(2) (4), and bis[(dicarboxymethyl)dithiocarbamato]copper(II), Cu(IdadtC)(2) (5), with nitric oxide in aqueous solution at pH 7.4 and 20 degrees C was spectrophotometrically studied and the experimental data were analysed by means of the SPEFO-LETAGROP and HYPERQUAD programs, respectively. The stability constants of the complexes CuL2(NO) and CuL2(NO)(2) [L = dithiocarbamate] were determined. The interaction of 1 and 2 with nitric oxide was theoretically studied by molecular mechanics methods. Complexes 3, 4 and 5 and the previously studied bis[(dihydroxyethyl)dithiocarbamato]copper(II), Cu(Deadtc)(2) (6), decrease the NO produced in vitro either by sodium nitroprusside (NPS) or by cultured murine macrophages J774 stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN gamma). Inducible nitric oxide synthase (iNOS) activity was determined by measuring the stable NO end product, nitrite, using a colorimetric assay. (c) 2006 Elsevier Ltd. All rights reserved.
机译:制备了三种新的双(二硫代氨基甲酸酯)铜(II)配合物,并通过元素分析,紫外-可见光谱和红外光谱进行了表征:双(4-哌啶酮二硫代氨基甲酸酯)铜(II),[Cu(PdtC)(2)](2 )(1),双(哌啶二硫代氨基甲酸酯)铜(II),[Cu(PpdtC)(2)](2)(2)和双[(2-哌啶基羧基)二硫代氨基甲酸酯]铜(II),Cu(Ppidtc)( 2)(3)。配合物I和2的晶体结构已经使用X射线衍射法确定,并且它们是二聚体。铜(II)离子的配位球被描述为扭曲的方锥:1和2的轴向Cu-S键距分别为2.7和2.8埃,比赤道Cu-S的键合距离(2.3埃)更长。配合物I和2不溶于水。相反,3是水溶性的。 31种先前制备的双[N-(二硫代羧基)肌氨酸]铜(II),Cu(Sdtc)(2)(4)和双[(二羧基甲基)二硫代氨基甲酸酯]铜(II),Cu(IdadtC)( 2)(5),用一氧化氮在pH 7.4和20摄氏度的水溶液中分光光度法研究,并分别通过SPEFO-LETAGROP和HYPERQUAD程序分析实验数据。确定了配合物CuL2(NO)和CuL2(NO)(2)的稳定常数[L =二硫代氨基甲酸酯]。通过分子力学方法对1和2与一氧化氮的相互作用进行了理论研究。配合物3、4和5以及先前研究的双[((二羟乙基)二硫代氨基甲酸酯]铜(II),Cu(Deadtc)(2)(6)可降低硝普钠(NPS)或培养的鼠体外产生的NO脂多糖(LPS)和干扰素-γ(IFNγ)刺激巨噬细胞J774。诱导型一氧化氮合酶(iNOS)活性是通过使用比色测定法测量稳定的NO终产物亚硝酸盐来确定的。 (c)2006 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号