摘要:In this paper epithelial cell necroses(apoptosis)of mouse intestinal crypts induced by βRays fromtritiated water(HTO)was reported.The resultsshowed that the number of apoptotic cells perintestinal crypt 20 hrs after injection of
摘要:A preliminary study on application of Pluorometric Analysis ofDNA Unwinding(FADU)to the detection of DNA strand breaksinduced by indirect environmental mutagen benzo(a)pyrene(BaP)was conducted.The results showed that BaP,activated by PCB-in-duced rat liver S9mixture,could result in obvious increase of DNAstrand breaks in human peripheral leukocytes incubated with
摘要:With the high-speed and reliable inducedmethod of the reactionary original bacteriophageof SOS,we have studied the mutagenicity for 14species of the antineoplastic drugs in the mostcommon use and the suitable concentration-fieldsbeing used as the positive control-agents.Theresults obtained are as follows:
摘要:This paper reports that,by using SOS inductest,we testedthe inhibitory effects of many materials(such as tea,tumericOil,11 kinds of vegetables,and 14 kinds of vitamins)to themutational specificity of 6 kinds of antineoplastic drugs.
摘要:The high-speed screening of the antimutagen and mutagen isof a great importance for controlling carcinogenic factorsof environment and the cancer prevention.Therefore,westudied 4 high-speed synchro-test methods of the
摘要:The 19 strains of Panicillium cyclopium were isolatedfrom the cereals of Linxian were cultured in Raulin-Thomliquid medium,25℃ incubated 14 daies still condition.The liquids and membrans of the cultures were extractedrespectively.the extracts were examinated in 4 mutationtasts as follows.
摘要:Flos Carthami,one of the herbs which iswidely used,was found to cause mutation of E.coliND-160.In our research,the micronucleus test wasused to examine,the mutagenicity of two extractsof Flos Carthami on the mouse bone marrow PCEs andhuman peripheral tymphocytes.The results showedthat the rate of micronucleus of the mouse bonemarrow PCEs was enchanced by the water and
摘要:The carcinogenic effect of BaP on the re-spiratory system have been extensively inves-tigated.It has not been reported that thehuman respiratory tract(HRT)would undergopreneoplastic lesions experimentally afterexposure to BaP,because of being in diffi-culty to obtain the studying materials ofnormal HRT.
摘要:Our study employed micronucleus test in mouse bonemarrow cells to detect the genotoxicity of a crudeChinese herb:the water-extract of Tripterygiumhypoglaucum(Level.)Hutch(THH)which has been usedfor treatment of some human immune diseases.Eachgroup included 5 male and 5 female treated mice and2 animals with both sex for control group.The
摘要:Pregnant mice were irradiated with exponentialdecreas dose of tritiumβ-rays to investigatethe effect ofβ-radiation on 12 and 15 daysembryonic melanoblasts.The effect was studiedby scoring changes in the differentiation ofmelanocytes in the hair follicles of
摘要:Our studies about the sequential testing programfor predicting and identificating carcinogens,sequ-ential discriminant method and cost-effectivenessanalysis are summarized in this paper.We recommendthat the chemical under study is first detected inshort-term tests(STT)and calculated its probabilityof carcinogenicity(PC).if PC≥0.50 or≤0.03,thechemical should be discriminated as a
摘要:The mutagenicty and antimutagenicity potentials of three kinds ofdetergents,Aliphatic ethoxylate(AEO);Linear alkylbenzene sul-phonates with 7-carbons chain(LAS1);and LAS with 9-carbons chain(LAS2)were tested with three short-term bioassay.
摘要:The effect of fermented milk on micronuclei andapoptosis induced by DimethyIhydraziine(DMH)in thecolon crypt cells of mouse was studied.Fermented milk,milk and tap water were fed to three groups of C57BL mice.The animals were given DMH i.p.at a dosage of 20mg/kg onthe 7th day.The animals were sacrificed 24 hours
摘要:The purpose of the present study is to ascertainthe correlation between the different ionic radiusof rare earths nuclides such as Tm、Eu、Pmand its retention peculiarity as well as inductionof mutagenic effect on bone marrow cells.Our studyshowed that the accumulation peculiarity of rareearths nuclides will vary according to the
摘要:Several risk factors have been nominated for theirsuspected roles in the etiology of oesophageal neopla-sia in north-east of Iran;Mazandaran Province,whichhas shown one of the highest incidence rate in theworld.However,the attention is mostly focused on therole of diet,The commonly consumed fish,meat,vege-
摘要:The methods of in vitro and in vivo-in vitro combinationassay for autoradiographic detection of unscheduled DNAsynthesis(UDS)induced by chemical carcinogens wasestablished.To estimate the reliability of these assays,UDS in tissues slices was measured with the establishedmethods after exposure to MNNG and dimethylnitrosamine(DMN)in vitro or in vivo respectively.To study thesusceptibility of various tissues to chemicals,UDS
摘要:MutaMouse ia a transgenic CD2 mouse,homozygous fora bacterial Lacz gene stably integrated within alambda gt10 vector.The vector exists as a concatam-er of 2 x 40 copies at insertion sites on bothparental chromosomes.The vector is rescued at highefficiency from the genomic DNA of any tissue and ispackaged in vitro with a commercially available,
摘要:Biomonitoring with cytogenetic methods consistto compare a known or suspected exposed groups with acontrol group with different endpoints.Generallyfactors which may influence the baseline levels ofsister chromatid exchanges frequency fall in 2 cate-gories:technical and biological factors.In additionto age,sex,drug use,smoking habits,health statusmight also be relevant.During a long term survey ofpeople professionally exposed to ethylene oxide
摘要:Biomonitoring human exposed to alkylating agents with theSister Chromatid Exchanges assay(SCEs)and especially theHigh Frequency Cells concept(HFCs)is considered as asensitive approach for detecting a personal exposure.Six yearsafter a two-year chronic exposure of primates to EtO,a highlymutagenic agent,an persistently increased SCEs frequency hasrecently been published.In this experiment,the appearance of ahighly mutated subpopulation of cells(the High Frequency
摘要:Ten workers from a cyclotron unit have beenaccidentally exposed to Osmium tetroxide emanations during a fewhours.In vitro,Osmium tetroxide forms an irreversible bindingwith thymidine without apparently disturbance in the DNAstructure.
摘要:Since the discovery of chromosomal abnormalitiesspecifically linked to particular types of leukemia,cytogeneticstudies have been shown to be of great contribution not only forthe diagnosis of leukemia but also for prognosis of patients.Situation is less clear for patients with lymphoma for whichcells are generally more difficult to grow in culture.