Fumonisin B1 — Isolation and analytical characterisation for the application as reference material

摘要

A procedure for the production of Fumonsin B1 with a purity of more than 97% has been worked out. The isolated material was characterised by NMR and GC-MS and showed good agreement to literature data, thus proving the identity of the analysed FB1. For the purity assessment with LC-FLD and LC-MS a commercially available standard and the isolated FB1 sample were analysed under the same conditions. The overall purity of the FB1 samples was estimated as 91.9±8.1% (reference) and 97.6±2.4% (biopure), respectively.