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首页> 外文期刊>European review for medical and pharmacological sciences. >Expression of miR-29 and STAT3 in osteosarcoma and its effect on proliferation regulation of osteosarcoma cells
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Expression of miR-29 and STAT3 in osteosarcoma and its effect on proliferation regulation of osteosarcoma cells

机译:miR-29和Stat3在骨肉瘤中的表达及其对骨肉瘤细胞增殖调节的影响

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OBJECTIVE: STAT3 has been shown to be involved in the occurrence, progression, and resistance of various tumors. The abnormal expression of miR-29 is associated with the pathogenesis of osteosarcoma. The bioinformatics analysis showed a targeting relationship between miR-29 and STAT3 3’-UTR. This study investigated whether miR-29 regulates the STAT3 expression and affects the proliferation and apoptosis of osteosarcoma cells. PATIENTS AND METHODS: The tumor tissues of osteosarcoma patients were collected, and the adjacent tissues were used as controls to detect the expression of miR-29 and STAT3. The Dual-Luciferase Gene Reporter Assay validated the regulatory relationship between miR-29 and STAT3. The expression of miR-29 and STAT3 in normal osteoblasts hFOB1.19, osteosarcoma SJSA-1, and MG-63 was measured. SJSA-1 cells were divided into miR-NC group and miR-29 mimic group. Cell apoptosis and proliferation were detected by flow cytometry. RESULTS: Compared with adjacent tissues, miR-29 expression was decreased, and STAT3 expression was increased in osteosarcoma. There was a targeted regulation relationship between miR-29 and STAT3. Compared with hFOB1.19 cells, miR-29 expression in osteosarcoma SJSA-1 and MG-63 cells was decreased, with increased STAT3 expression. The transfection of miR-29 mimic significantly decreased the expression of STAT3 and p-STAT3 in SJSA-1 cells, inhibited cell proliferation, and increased cell apoptosis. CONCLUSIONS: Decreased miR-29 expression plays a role in the increase of the STAT3 expression and in the promotion of the pathogenesis of osteosarcoma. Increasing the expression of miR-29 can inhibit the proliferation of osteosarcoma cells and promote apoptosis by decreasing STAT3 expression.
机译:目的:Stat3已被证明参与各种肿瘤的发生,进展和抗性。 miR-29的异常表达与骨肉瘤的发病机制有关。生物信息学分析显示MIR-29和Stat3 3'-UTR之间的靶向关系。本研究调查了MIR-29是否调节STAT3表达并影响骨肉瘤细胞的增殖和凋亡。患者和方法:收集了骨肉瘤患者的肿瘤组织,并使用相邻组织作为对照检测miR-29和Stat3的表达。双荧光素酶基因记者测定验证了MIR-29和Stat3之间的监管关系。测定了MiR-29和Stat3在正常成骨细胞中的表达,骨质细胞HFOB1.19,骨肉瘤SJSA-1和MG-63。将SJSA-1细胞分为miR-NC组和MiR-29模拟组。通过流式细胞术检测细胞凋亡和增殖。结果:与相邻组织相比,MiR-29表达降低,骨肉瘤中的表达增加了STAT3表达。 MiR-29和Stat3之间存在有针对性的监管关系。与HFOB1.19细胞相比,随着STAT3表达增加,降低了骨肉瘤SJSA-1和MG-63细胞中的miR-29表达。 MiR-29的转染显着降低了SJSA-1细胞中的STAT3和P-STAT3的表达,抑制细胞增殖和增加的细胞凋亡。结论:下降miR-29表达在STAT3表达的增加和促进骨肉瘤的发病机制中起着作用。提高miR-29的表达可以通过减少STAT3表达来抑制骨肉瘤细胞的增殖并促进细胞凋亡。

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