Multicenter Evaluation of a Semiautomated, Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations

Luisa Romanò; Claudio Velati; Lorella Baruffi; Laura Fomiatti; Giuseppe Colucci; Alessandro R. Zanetti

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Multicenter Evaluation of a Semiautomated, Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations

机译：用于献血中乙型肝炎病毒DNA检测的半自动化标准化检测方法的多中心评估

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We evaluated the COBAS Ampliscreen hepatitis B virus (HBV) test using standards, seroconversion panels, consecutive donations, and samples from patients with abnormal alanine aminotransferase and chronic hepatitis C. Specificity was 100% and sensitivity was 20 IU/ml. In seroconversion panels, HBV DNA was detected up to 4 to 18 days before HBsAg, suggesting that this assay is useful in shortening the infectious window phase.

机译：我们使用标准品，血清转换小组，连续捐赠以及来自异常丙氨酸转氨酶和慢性丙型肝炎患者的样品评估了COBAS Ampliscreen乙型肝炎病毒（HBV）测试。特异性为100％，敏感性为20 IU / ml。在血清转化组中，在HBsAg发生之前的4至18天检测到HBV DNA，这表明该测定法可用于缩短感染窗口期。

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《Journal of Clinical Microbiology》 |2005年第6期|共3页
作者
Luisa Romanò; Claudio Velati; Lorella Baruffi; Laura Fomiatti; Giuseppe Colucci; Alessandro R. Zanetti;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Multicenter performance evaluation of a transcription-mediated amplification assay for screening of human immunodeficiency virus-1 RNA, hepatitis C virus RNA, and hepatitis B virus DNA in blood donations. [J] . Koppelman MH, Assal A, Chudy M, Transfusion: The Journal of the American Association of Blood Banks . 2005,第8期

机译：用于筛选献血中人类免疫缺陷病毒-1 RNA，丙型肝炎病毒RNA和乙型肝炎病毒DNA的转录介导扩增测定的多中心性能评估。
2. Dynamic Range and Reproducibility of Hepatitis B Virus (HBV) DNA Detection and Quantification by Cobas Taqman HBV, a Real-Time Semiautomated Assay [J] . Magnus Lindh, Charles Hannoun Journal of Clinical Microbiology . 2005,第8期

机译：实时半自动测定法Cobas Taqman HBV检测和定量乙肝病毒（HBV）DNA的动态范围和可重复性
3. Multicenter evaluation of a commercial multiplex polymerase chain reaction test for screening plasma donations for parvovirus B19 DNA and hepatitis A virus RNA [J] . KoppelmanM.H.G.M., CuijpersH.T.M., WessbergS., Transfusion: The Journal of the American Association of Blood Banks . 2012,第7aPta1期

机译：用于筛选细小病毒B19 DNA和甲型肝炎病毒RNA血浆捐赠物的商业多重聚合酶链反应测试的多中心评估
4. AN INTEGRATED MICROCANTILEVER-BASED WIRELESS DNA CHIP FOR HEPATITIS B VIRUS (HBV) DNA DETECTION [C] . Che-Wei Huang, Yu-Jie Huang, Tsung-Hsien Lin, International Conference on Miniaturized Systems for Chemistry and Life Sciences . 2011

机译：用于乙型肝炎病毒（HBV）DNA检测的基于集成的微膜无线DNA芯片
5. Chimeric orthohepadnavirus core particles for oral delivery of vaccines: Part I. Transformation of tobacco plants with a gene encoding a c-terminus truncated hepatitis B virus core protein. Part II. Construction of a woodchuck hepatitis virus core protein-based universal epitope carrier and test expression in Escherichia coli. [D] . Callaghan, Maximilian W. 2001

机译：用于口服递送疫苗的嵌合正丙肝病毒核心颗粒：第一部分。用编码c末端截短的乙型肝炎病毒核心蛋白的基因转化烟草植物。第二部分基于土拨鼠肝炎病毒核心蛋白的通用表位载体的构建和大肠杆菌中的表达测试。
6. Multicenter Evaluation of a Semiautomated Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations [O] . Luisa Romanò, Claudio Velati, Lorella Baruffi, 2005

机译：多中心评估用于献血中乙型肝炎病毒DNA检测的半自动化标准化测定
7. Multicenter Evaluation of a Semiautomated, Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations [O] . Romanò, Luisa, Velati, Claudio, Baruffi, Lorella, 2005

机译：多中心评估用于献血中乙型肝炎病毒DNA检测的半自动化标准化测定

Multicenter Evaluation of a Semiautomated, Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations

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