Improved PCR for Detection of the Highly Leukotoxic JP2 Clone of Actinobacillus actinomycetemcomitans in Subgingival Plaque Samples

摘要

The JP2 clone of Actinobacillus actinomycetemcomitans is associated with early-onset periodontitis in certain ethnic populations of African origin. Here, we describe and evaluate a set of primers for PCR to assay for the presence of A. actinomycetemcomitans and to discriminate between JP2-like strains and other genotypes in subgingival plaque samples.