首页> 外文期刊>BMC Genomics >Impact of selenium supplementation on fish antiviral responses: a whole transcriptomic analysis in rainbow trout ( Oncorhynchus mykiss ) fed supranutritional levels of Sel-Plex?
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Impact of selenium supplementation on fish antiviral responses: a whole transcriptomic analysis in rainbow trout ( Oncorhynchus mykiss ) fed supranutritional levels of Sel-Plex?

机译:硒对鱼类抗病毒反应的影响:补充营养水平的Sel-Plex对虹鳟(Oncorhynchus mykiss)的整个转录组学分析?

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Selenium (Se) is required for the synthesis of proteins (selenoproteins) with essential biological functions. Selenoproteins have a crucial role in the maintenance of cellular redox homeostasis in nearly all tissues, and are also involved in thyroid hormone metabolism, inflammation and immunity. Several immune processes rely on Se status and can be compromised if this element is present below the required level. Previous work has supported the notion that when Se is delivered at levels above those deemed to be the minimal required but below toxic concentrations it can have a boosting effect on the organism’s immune response. Based on this concept Se-enriched supplements may represent a valuable resource for functional feeds in animal farming, including aquaculture. In this study we tested the effects of Se supplemented as Sel-Plex during an immune challenge induced by polyinosinic:polycytidylic acid (poly(I:C)), a pathogen-associated molecular pattern (PAMP) that mimics viral infection. Trout were fed two diets enriched with 1 or 4?mg Se Kg?1 of feed (dry weight) by Sel-Plex addition and a commercial formulation as control. The whole trout transcriptomic response was investigated by microarray and gene ontology analysis, the latter carried out to highlight the biological processes that were influenced by Sel-Plex supplementation in the head kidney (HK) and liver, the main immune and metabolic organs in fish. Overall, Sel-Plex enrichement up to 4?mg Se Kg?1 induced an important response in the trout HK, eliciting an up-regulation of several genes involved in pathways connected with hematopoiesis and immunity. In contrast, a more constrained response was seen in the liver, with lipid metabolism being the main pathway altered by Se supplementation. Upon stimulation with poly(I:C), supplementation of 4?mg Se Kg?1 increased the expression of principal mediators of the antiviral defences, especially IFN-γ, and down-stream molecules involved in the cell-mediated immune response. Supplementation of diets with 4?mg Se Kg?1 using Sel-Plex remarkably improved the fish response to viral PAMP stimulation. Sel-Plex, being a highly bioavailable supplement of organic Se, might represent a suitable option for supplementation of fish feeds, to achieve the final aim of improving fish fitness and resistance against immune challenges.
机译:硒(Se)是合成具有基本生物学功能的蛋白质(硒蛋白)所必需的。硒蛋白在维持几乎所有组织的细胞氧化还原稳态中起着至关重要的作用,并且还参与甲状腺激素的代谢,炎症和免疫。几种免疫过程取决于硒的状态,如果该元素的含量低于所需水平,则可能会受到损害。先前的工作支持以下观点:当硒的释放量高于被认为是最低需求的水平,但低于有毒浓度时,则会对生物体的免疫反应产生促进作用。基于这一概念,富硒补品可能是动物养殖包括水产养殖中功能性饲料的宝贵资源。在这项研究中,我们测试了在多肌苷酸:聚胞苷酸(poly(I:C))(一种模拟病毒感染的病原体相关分子模式(PAMP))诱导的免疫攻击过程中,添加硒作为Sel-Plex的效果。通过添加Sel-Plex和市售配方作为对照,向鳟鱼喂食两种富含1或4 mg Se Kg?1饲料(干重)的日粮。通过微阵列和基因本体分析研究了整个鳟鱼的转录组反应,后者的目的是强调鱼类的主要免疫和代谢器官头部肾脏(HK)和肝脏中受Sel-Plex补充影响的生物学过程。总体而言,Sel-Plex富集至4?mg Se Kg?1会在鳟鱼HK中引起重要反应,从而引起与造血和免疫相关的通路中涉及的几个基因的上调。相反,在肝脏中观察到更受限的反应,脂质代谢是补充硒改变的主要途径。受到聚(I:C)刺激后,添加4?mg Se Kg?1可以增加抗病毒防御素(尤其是IFN-γ)的主要介体和参与细胞介导的免疫反应的下游分子的表达。使用Sel-Plex补充4 mg Se Kg?1的饮食可以显着改善鱼类对病毒PAMP刺激的反应。 Sel-Plex是一种高生物利用度的有机硒补充剂,可能是补充鱼类饲料的合适选择,以实现提高鱼类适应性和抵抗免疫挑战的最终目的。

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