Direct Elisa Kit for the detection of Flavobacterium psychrophilum in internal organs of rainbow trout (Oncorhynchus mykiss)

摘要

Refers to a direct Elisa Kit for determining the presence of Flavobacterium psychrophilum in internal organs (Kidney and spleen) of rainbow trout (Oncorhynchus mykiss), comprising: (a) microplate Wells; (b) Antibodies of Mouse antiu2013flavobacterium psychrophilum NCIMB 1947Where these Antibodies are conjugated with Horseradish Peroxidase (HRP) as a Tracer Enzyme; and (c) a Solution containing tetrametilbenzidina (TMB) as a substrate on which the Enzyme acts HRP.It also refers to methods for determining the presence of Flavobacterium psychrophilum in internal organs of Rainbow Trout with Elisa kit of the present Invention, and to methods for obtaining Antibodies antiu2013flavobacterium psychrophilum NCIMB 1947 from Mouse Serum and Conjugation with HRP