Gas Chromatographic Assessment for Bioremediation of Hydrocarbons pollutants Using Bacillus Amyloliquefaciens

摘要

The bioremediation of n-Octane and Ethyl benzene singly and in combination as representative solutes for aliphatic and aromatic hydrocarbons using Bacillus amyloliquefaciens was studied. The studied B. amyloliquefaciens was selected from one hundred and sixteen bacterial isolates collected from five different sites along River Nile, Egypt. The determination of the minor amounts of the residual selected solutes was obtained via capillary gas chromatography (CGC) using normal hexane as internal standard. The effects of different pH values, incubation temperatures and the exposure of the bacterial cells to 7.3mW He-Ne laser radiation on the bioremediation were investigated. PH 6 and incubation temperature 37 oC are the optimum conditions giving maximum degradation of both aliphatic and aromatic hydrocarbons. Complete degradation through incubation time 12 hours was obtained by irradiating the bacterial cells to a laser dose for one minuet. The irradiation for three minuets exhibits complete degradation through incubation time 8 hours only. Irradiation of the bacterial suspension for more than three minuets accompanied with bad effect on the bioremediation process.