Genetic polymorphism of five genes associated with growth traits in goat

摘要

Genetic polymorphism studies in domestic animals aim at evaluating genetic variations within and across breeds mainly for conservation purposes. In this study, polymerase chain reaction-restriction fragment length polymorphism?(PCR-RFLP) was used to detect polymorphisms of five candidate genes in four Egyptian and Saudi goat breeds (Barki, Zaribi, Ardi and Masri), to detect the genotype of GH, IGF1, POUIF1, MSTN?and BMP15 genes in the goat breeds and their allele frequencies. Results of GH gene which encloses a Haelll endonuclease restriction site show four unique PCR-RFLP banding patterns (genotypes AA, AB, CC and CD). The frequencies of the A allele in the samples from the goat breeds varied from 0.410 to 0.620. While IGF-1gene revealed three fragments after digestion with Haelll with genotype AA, AB and BB and the frequencies of allele A varied from?0.432 to 0.731.Furthermore,?PCR-RFLP of POUIF1?gene showed two fragments after digestion?by Pst1 endonuclease with genotype TT and CC and the frequencies of allele T varied from?0.250 to 0.840. The?MSTN gene revealed three fragments?after digestion withDraI?with genotype AA, BB and AB and the frequencies of allele A?varied from?0.240 to 0.630. Meanwhile, the?BMP15 gene revealed one fragments of 112 bp for AA after digestion with Hinf1?enzyme.