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Extraction and characterization of gelatin from camel skin (potential halal gelatin) and production of gelatin nanoparticles

机译:从骆驼皮中提取和表征明胶(潜在的清真明胶)并生产明胶纳米颗粒

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摘要

Gelatin is used as an ingredient in both food and non-food industries as a gelling agent, stabilizer, thickener, emulsifier, and film former. Porcine skins, bovine hides, and cattle bones are the most common sources of gelatin. However, mammalian gelatins are rejected by some consumers due to social, cultural, religious, or health-related concerns. In the present study, gelatin was obtained from camel skin as an alternative source using a combination of processing steps. Central composite design combined with response surface methodology was used to achieve high gelatin yields under different extraction conditions: temperatures of 40, 60, and 80 °C; pH values of 1, 4, and 7; and extraction times of 0.5, 2.0, and 3.5 min. Maximum gelatin yield from camel skin (29.1%) was achieved at 71.87 °C and pH 5.26 after 2.58 min. The extracted gelatin samples were characterized for amino acid profile, foaming capacity, film formation, foam stability, and gel strength (Bloom value). Gelatin nanoparticles were produced, and their morphology and zeta potential were determined. Bloom value of the camel skin gelatin was 340 g. Amino acid analysis revealed that the extracted gelatin showed high glycine and proline contents. Analysis of camel skin gelatin nanoparticle and functional properties revealed high suitability for food and non-food applications, with potential use in the growing global halal food market.
机译:明胶在食品和非食品工业中均用作胶凝剂,稳定剂,增稠剂,乳化剂和成膜剂。猪皮,牛皮和牛骨头是明胶的最常见来源。但是,由于社会,文化,宗教或健康方面的考虑,某些消费者拒绝使用哺乳动物明胶。在本研究中,使用加工步骤相结合,从骆驼皮获得明胶作为替代来源。使用中央复合材料设计与响应表面方法相结合,在不同的提取条件下(40、60和80°C的温度)获得高明胶产量。 pH值为1、4和7;提取时间为0.5、2.0和3.5分钟。 2.58分钟后,在71.87°C和pH 5.26下,骆驼皮的最大明胶产量(29.1%)。提取的明胶样品的氨基酸特征,起泡能力,成膜性,泡沫稳定性和凝胶强度(布鲁姆值)均经过表征。制备明胶纳米颗粒,并确定其形态和ζ电势。骆驼皮明胶的布卢姆值是340 g。氨基酸分析表明,提取的明胶显示出高的甘氨酸和脯氨酸含量。对骆驼皮明胶纳米颗粒和功能特性的分析显示,它们非常适合食品和非食品应用,并且在不断增长的全球清真食品市场中具有潜在的用途。

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