南天竹两品种的组培快繁技术

摘要

以火焰南天竹和尖叶南天竹茎段与茎尖为外植体，研究不定芽诱导、增殖和生根技术。结果表明，两品种的最佳增殖培养基均为MS＋6-BA 1.0 mg·L-1＋NAA 0.1 mg·L-1；在培养基1/2MS＋NAA 0.1 mg·L-1＋IBA 0.1 mg·L-1中，尖叶南天竹生根率可达100%，而火焰南天竹生根率仅73.5%；将火焰南天竹的生根材料在该生根培养基中接种后培养15 d，再转入经γ射线辐射灭菌的椰糠中，可将生根率提高至92.0%，且植株生长健壮。%The stems and stem apexes ofNandina domestica ‘Firepower’ andN. domestica ‘Harbour Dwarf’ were used as explants to study the techniques of adventitious bud induction, proliferation and rooting. The results showed that the best medium for proliferation of two cultivars was MS + 6-BA 1.0 mg·L-1+ NAA 0.1 mg·L-1. The rooting rate ofN. domestica ‘Harbour Dwarf’ could be as high as 100% in rooting medium 1/2MS + NAA 0.1 mg·L-1+ IBA 0.1 mg·L-1, while only 73.5% for the other cultivar. Plantlets in propagation stage were cultivated for 15 days in the root medium, and then transplanted to coconut chaff in plastic containers which were sterilized byγ-ray. In the coconut chaff, the rooting rate of ‘Firepower’ could be increased to 92.0%, and the plantlets grew well.