心脏缺血再灌注大鼠Th1/Th2细胞平衡与心脏微血管fgl2表达的相关性

摘要

目的 探讨大鼠Th1/Th2细胞平衡与心脏微血管fgl2表达是否存在相关性.方法 将雄性大鼠随机分成正常对照组、假手术组和模型组,模型组又分为缺血再灌注3 h、6 h、24 h、3 d、7 d五个亚组,采用实时荧光定量PCR方法检测各组缺血再灌注区心脏组织fgl2 mRNA的表达,ELISA方法检测血清IFN-γ、IL-4水平,以IFN-γ/IL-4比值代表Th1/Th2平衡状况,免疫组化方法检测心脏微血管中fgl2的表达.结果 正常对照组与假手术组fgl2表达、Th1/Th2比值均无统计学差异;模型组fgl2表达、Th1/Th2比值均显著高于正常对照组和假手术组(P均＜0.05),且于再灌注6 h达高峰.免疫组化结果显示,心脏微血管缺血再灌注3 h即有fgl2沉积,再灌注6 h沉积最明显,之后逐渐减少.Spearman等级相关分析显示,Th1/Th2与fgl2水平呈显著正相关(r=0.867,P＜0.05).结论 Th1/Th2平衡状态与心脏微血管fgl2表达存在相关性.%Objective To explore the relationship between Th1/Th2 imbalance and the microvasculature fgl2 expression existed in rat heart I/R model. Methods Male SD rats were divided into three groups, control group, sham-operated group and operation-treated group, randomly. The operation-treated group was divided five subgroups after I/R 3 h, 6 h, 24 h, 3 d, 7 d.The rats were sacrificed at the corresponding time point. Real-time Sybr Green ⅠPCR tested the expression of the microvasculature fgl2-mRNA. Serum cytokines IFN-γ and IL-4 were tested with the ELISA kits, the ratio of IFN-γ/IL-4 represented the balance of Th1/Th2. S-P immunohistochemistry assay detected fgl2 expression in the microvasculature. Results There was no significant difference between control group and sham-operated group in the expression of microvasculature fgl2,the expression of microvasculature fgl2 in operation-treated group was significant higher than that in control group and sham-operated group(all P＜0.05),the expression of fgl2 attained the peak at the 6 h after I/R. There was no significant difference in Th1/Th2 ratio between the control group and the sham-operated group. The Th1/Th2 ratio of operation-treated group was significant higher than that of control group and sham-operated group(all P＜0.05),the ratio attained the peak at the 6h after I/R. Fgl2 could be deposited at the 3 h, and attained the peak at the 6 h after I/R, decreased gradually after the point time. There was a significant positive correlation existing between the ratio of Th1/Th2 and the expression of microvasculature fgl2(r=0.867,P＜0.05).Conclusion The positive relationship existed between Th1/Th2 imbalance and the microvasculature fgl2 expression.