大鼠酒精性脂肪肝两种建模方法的比较

摘要

目的 比较生理途径诱导与乙醇灌胃建立大鼠酒精性脂肪肝模型的差异.方法 雄性SD大鼠随机分成对照组、饮用乙醇、乙醇灌胃组,12周后测定大鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰基转移酶(γ-GT)水平,并观察大鼠肝脏、胃肠组织形态学改变.结果 12周后饮用乙醇组与乙醇灌胃组大鼠AST、γ-GT水平、肝指数与对照组比较差异具有统计学意义(P均＜0.05),且肝组织出现大量脂肪变性,伴有局部炎症和坏死;饮用乙醇组与乙醇灌胃组ALT、AST、γ-GT、肝指数差异无统计学意义,肠胀气发生率差异具有统计学意义(P＜0.01).结论 采用生理途径诱导大鼠酒精性脂肪肝动物模型更具有应用价值.%Objective To compare establishment of experimental model of chronic alcoholic fatty liver in rats by physiological path and ethanol intra gastric infusion. Methods The male SD rats were divided into three groups, namely control group, drinking alcohol group, ethanol intra gastric infusion group. ALT, AST, γ-GT activity were detected after 12 weeks, and observing the morphologic change of liver and intestines and stomach tissue in rats. Results After 12 weeks,The AST activity, γ-GT levels,liver indexes in drinking alcohol group were statistically different with that in ethanol intra gastric infusion group ( all P ＜ 0.05 ), in which local inflammation and necrosis were existed. There was no statistical significance in ALT, AST, γ-GT, liver indexes, and had statistical significance in gastric bowel bilges gas between drinking alcohol group and ethanol intra gastric infusion group (P ＜0.05). Conclusions The experimental animal model of chromic alcoholic fatty liver is succesfully established by ethanol intragastric infusion or drinking alcohol. The latter is more worthy of application.