重组人血管内皮抑制素对多发性骨髓瘤内皮细胞增殖和迁移的影响

摘要

Objective To observe the effect of inhibiting proliferation and inducing apoptosis effect of recombinant human endostatin(ES) on the human umbilical vein endothelial cells ( HUVECs) and myeloma cell lines KM3, and investigate its influence on HUVEC immigration induced by KM3 cell cultured supernatant. Methods Cell proliferation in different concentration of ES was determined by WST-1 assay. The fluorescence flow cytometry ( FCM) Annexin V-FITC assay was applied to detect the changes of apoptotic cells. Modified Boyden chamber assay was used to observe the migration activity of HUVEC induced by KM3 cells. Results 50-500 ng/mL ES inhibited HUVEC proliferation, but had no influence on KM3 cell lines. 100, 200 ng/mL ES induced HUVEC apoptosis, but had no effect on KM3 cell lines. ES inhibited the endothelial cell migration induced by KM3 cell cultrued supernatant(P<0.01). Conclusion ES can not only inhibit the proliferation and induce apoptosis of HUVEC cells but also inhibit endothelial cell migration ability of multiple myeloma cells.%目的 观察重组人血管内皮抑制素注射液(内皮抑素)对人脐静脉内皮细胞株(HUVEC)和多发性骨髓瘤细胞株KM3抑制增殖及诱导凋亡的作用,研究其对KM3细胞培养上清诱导的HUVEC迁移效果的影响.方法 WST-1法观察不同浓度的内皮抑素分别对HUVEC和KM3细胞株的增殖抑制作用,Annexin V-FITC法检测有效浓度的内皮抑素对HUVEC和KM3细胞株的凋亡作用,改良的Boyden小室法检测内皮抑素对KM3细胞株培养上清诱导的HUVEC迁移的影响.结果 50 ～ 500 ng/mL内皮抑素对HUVEC具有抑制增殖作用,而对KM3细胞未见明显作用.100、200 ng/mL的内皮抑素对HUVEC具有诱导凋亡作用;而对KM3细胞未见明显作用.与不加药组相比,内皮抑素能抑制KM3细胞培养上清促内皮细胞迁移的作用(P＜0.01).结论 内皮抑素能抑制HUVEC的增殖及诱导其凋亡,并能抑制骨髓瘤细胞的促内皮细胞迁移作用.