乌梅丸对TNBS诱导的结肠炎大鼠脾脏组织Bcl-2表达的影响

摘要

Objective To observe the effect of Wumeiwan on the expression of Bcl-2 protein and mRNA in the spleen tissue of rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis and to investigate its immune mechanism in treating colitis.Methods Fifty-six SD rats were randomly divided into the control group,colitis model group,mesalazine group and Wumeiwan group (14 rats in each group).Apart from the control group,rats in the other three groups were induced to experimental colitis by 2,4,6-trinitrobenzenesulfonic acid.And 2 days later,rats in the control group and colitis model group were administered intragastrically with normal saline at a dose of 3 mL,while rats in the mesalazine group and Wumeiwan group were intragastrically given mesalazine (50 g/L) and Wumeiwan (0.515 g/L) at a dose of 3 mL,respectively.All rats were treated for 15 d.Spleen tissue samples were taken to detect the expression of mRNA of Bcl-2 by realtime PCR and the expression of Bcl-2 protein by Western blot.Results Compared with the control group,the expression of Bcl-2 mRNA and Bcl-2 protein was significandy increased in the colitis model group (all P ＜ 0.05) ; compared with the colitis model group,the expression of the mRNA and protein of Bcl-2 in the Wumeiwan and mesalazine groups was significantly decreased (all P ＜ 0.05).And there were no significant differences in those between the Wumeiwan group and mesalazine group (P ＞ 0.05).Conclusion Wumeiwan plays an important role in the treatment of rat TNBS-induced colitis by down-regulating the expression of the mRNA and protein of Bcl-2 in the spleen tissue.%目的 观察乌梅丸对2,4,6-三硝基苯磺酸(TNBS)诱导的结肠炎大鼠脾脏组织Bcl-2蛋白和mRNA表达的影响,研究其治疗结肠炎的免疫机制.方法 将56只SD大鼠随机分成空白对照组、结肠炎模型组、美沙拉嗪组、乌梅丸组各14只.除空白对照组外,其他三组均用TNBS灌肠.模型建成2d后,空白对照组和结肠炎模型组均以蒸馏水3mL灌胃,美沙拉嗪组用50 g/L美莎拉嗪混悬液、乌梅丸组用0.515 g/L乌梅丸液3mL灌胃,连续给药15 d后其取脾脏组织,应用Real time-PCR方法检测Bcl-2 mRNA表达,Western-blot技术检测Bcl-2蛋白表达.结果 与空白对照组相比,结肠炎模型组Bcl-2 mRNA和蛋白表达升高(P均＜0.05)；与结肠炎模型组相比,乌梅丸组、美沙拉嗪组Bcl-2 mRNA和蛋白表达下降(P均＜0.05),而乌梅丸组和美沙拉嗪组比较无统计学差异(P＞0.05).结论 乌梅丸通过下调结肠炎大鼠脾脏组织Bcl-2 mRNA和蛋白表达,发挥治疗结肠炎的作用.