目的：观察小白菊内酯对人肝癌细胞HepG-2细胞增殖、凋亡、迁移的影响。方法实验组将2．5、5、10、20、40μg/mL的小白菊内酯分别作用于HepG-2细胞，对照组不加小白菊内酯干预，MTT比色法观察小白菊内酯对HepG-2细胞生长增殖的影响，AO/EB及Hoechst33258染色法在荧光显微镜下观察细胞形态学的改变；用流式细胞仪技术检测小白菊内酯作用前后细胞周期的改变和细胞凋亡情况；用细胞划痕实验的方法检测小白菊内酯对细胞迁移的影响。结果小白菊内酯作用HepG-2后细胞增殖被抑制，随着小白菊内酯浓度逐渐增加和作用时间的延长，HepG-2细胞生长抑制率上升（P均＜0．05）；5 mg/L的小白菊内酯作用48 h后可见细胞呈明显的细胞形态学改变，胞质减少、细胞核染色质固缩，出现凋亡小体；实验组与对照组G0/G1期细胞所占比例分别为73．36％±9．13％、59．28％±8．37％，S期所占比例分别为18．34％±6．09％、27．36％±4．26％，G2/M期所占比例分别为9．36％±2．98％、14．30％±3．07％，凋亡率分别为27．45％±4．15％、0．56％±0．72％，两组比较，P＜0．05。实验组细胞迁移能力明显弱于对照组（P＜0．05）。结论小白菊内酯通过将细胞阻滞在G0/G1期而抑制HepG-2细胞增殖并诱导其凋亡；小白菊内酯对HepG-2细胞有明显的抗迁移作用。%Objective Objective To observe the effect of parthenolide on human hepatocellular carcinoma HepG -2 cell proliferation, apoptosis, migration.Method The parthenolide 2.5, 5, 10, 20, 40 μg/mL respectively in HepG-2 cells, MTT colorimetric assay was used to observe the effect ofparthenolide on growth and proliferation of HepG -2 cells, cell mor-phology was observed under a fluorescence microscope AO /EB and Hoechst33258 staining methods change;change and ap-optosis by flow cytometry before and after detection of parthenolide cell cycle;different methods cell scratch test of parthe-nolide on cell migratio.Result Parthenolide treated HepG-2 cell proliferation was inhibited (P<0.05), and in a time and dose dependent (P<0.05);5μg/mL parthenolide effect after 48 h visible cells showed morphological changes significant-ly, thecytoplasm reduced , nuclear chromatin pyknosis , appeared apoptotic bodies; G0/G1 cells number of parthenolide treated HepG-2 cell cycle in S phase increased , and the cell number in G 2/M phase was decreased ( P<0 .05 ); weaken the migration ability of parthenolide treated HepG-2 cells.Conclusion Parthenolide the cells arrest in G 0/G1 phase and in-hibit proliferation and induce apoptosis of HepG-2 cells;parthenolide has obvious anti migration effects on HepG-2 cells.
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