目的：探讨苦参碱对炎症状态下肾小管上皮细胞B7-H1 mRNA及蛋白表达的影响。方法取人肾小管上皮细胞（ HK-2）用于实验。①苦参碱浓度对B7-H1的影响：设对照组、模型组及苦参碱低、中、高剂量组，对照组仅加含5％胎牛血清的DMEM培养液，模型组及苦参碱低、中、高浓度组加入终浓度为100 g／L的IFN-γ，苦参素低、中、高浓度组再分别加入0．5、0．8、1．0 g／L的苦参碱；培养24 h，采用RT-PCR法检测HK-2细胞中的B7-H1 mRNA，流式细胞术检测B7-H1蛋白。②苦参碱干预时间对B7-H1的影响：设对照组、模型组及苦参碱不同培养时间组（12、16、24 h），对照组仅加含5％胎牛血清的DMEM培养液培养24 h，模型组加终浓度为100 g／L的IFN-γ培养24 h，苦参碱12 h、16 h、24 h组加入终浓度为100 g／L的IFN-γ和0．8 g／L的苦参碱，分别培养12、16、24 h，采用RT-PCR法检测HK-2细胞中的B7-H1 mRNA。结果模型组B7-H1 mRNA及其蛋白表达量高于对照组，苦参碱低、中、高浓度组低于模型组（P均﹤0．05）；苦参碱12 h、16 h、24 h组B7-H1 mRNA及其蛋白表达低于模型组（P均﹤0．05）。苦参碱抑制HK-2细胞B7-H1 mRNA表达作用呈剂量、时间依赖性，对B7-H1蛋白的抑制作用也呈剂量依赖性（P均﹤0．05）。结论 IFN-γ诱导的炎症状态下肾小管上皮细胞B7-H1 mRNA、蛋白表达增高，苦参碱可下调B7-H1 mRNA、蛋白表达。%Objective To explore the effect of matrine on expression of B 7-H1 in tubular epithelial cells .Methods①The effect on different dose of matrine:human HK-2 cells were cultured under five culture conditions to observe and di-vided into 4 groups, which was control group(5%fetal calf serum+DMEM for 24 h), model group (IFN-100 g/L for 24 h), matrine group (IFN-100 g/L+0.8 g/L matrine for 12 h,16 h and 24 h) .The expression of B7-H1 mRNA was evalu-ated by using real time RT-PCR.The surface expression of B7-H1 on HK-2 was analyzed by using flow cytometry .②The effect on different time of matrine: cell were divided into control group (5% fetal calf serum+DMEM for 24 h), model group (IFN-100 g/L for 24 h), matrine group (IFN-100 g/L+0.8 g/L matrine for 12 h,16 h and 24 h).The expression of B7-H1 mRNA was evaluated by using real time RT-PCR.Results The expression of B7-H1 on HK-2 cells was de-creased in normal condition .IFN-can significantly increased the expression of B 7-H1.Matrine decreased the B7-H1 ex-pression of HK-2 cells in a dose and time dependent way at mRNA levels and in a dose dependent way at protein levels ( P<0.05).Conclusion B7-H1 mRNA and protein increased under the condition of inflammation induced by IFN -γ.Ma-trine could down regulate the expression of B 7-H1 mRNA and protein .
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