儿童双侧肾母细胞瘤差异性表达蛋白的筛选

摘要

目的：采用表面增强激光解析电离飞行时间质谱（ SELDI-TOF-MS）技术筛选双侧肾母细胞瘤（ BWT）的差异性表达蛋白。方法选取BWT患儿血清标本10例（BWT组），另选单侧肾母细胞瘤（UWT）患儿血清标本（ UWT组）及健康儿童血清标本（对照组）各20例，采用SELDI-TOF-MS技术检测并收集相关数据。结果应用生物信息学方法对所得数据进行分析，得到差异性峰10个（ P＜0．01）。对比各组差异性表达蛋白峰值数据，筛选出质子数／电荷数（ m／z）为5648 Da的差异性表达蛋白1个，其在BWT 组、UWT组及对照组中表达强度分别为3889．36±1796．83、2886．81±1404．65、432．21±730．42，三组相比，P均＜0．01。结论采用SELDI-TOF-MS技术成功筛选出一种BWT差异性表达蛋白，其有望成为BWT早期诊断及预后判断的新的标志物。%Objective To select the differentially expressed proteins of bilateral wilms tumor (BWT) by using SELDI-TOF-MS technology .Methods A total of 10 serum samples of children with BWT ( BWT group ) , 20 serum samples of children with Unilateral Wilms Tumor ( UWT group ) and 20 serum samples of healthy children ( control group ) were select-ed.SELDI-TOF-MS technology was used to detect the differentially expressed BWT proteins .Results Ten difference BWT proteins peaks were found(P<0.01).The highest Youden′s index was obtained to select one differentially expressed protein with m/z of 5 648 Da.The selected expression BWT protein in BWT group , UWT group and control group was 3 889.36 ±1 796.83, 2 886.81 ±1 404.65 and 432.21 ±730.42, respectively(all P<0.01).Conclusion A differen-tially expressed protein of BWT group was successfully obtained by using SELDI-TOF-MS technology , which is expected to be a new marker for the early diagnosis and prognosis of BWT .