ErbB2基因rs1058808位点多态性与延边地区HBV相关肝细胞肝癌的关系及民族差异

摘要

目的 观察延边地区乙型肝炎病毒(HBV)相关肝细胞肝癌(HCC)患者ErbB2基因rs1058808位点多态性,探讨rs1058808位点多态性与HBV相关HCC的关系及民族差异.方法 选取66例(朝鲜族28例、汉族38例)HBV相关HCC患者(病例组)和150例(朝鲜族68例、汉族82例)单纯HBV携带者(对照组),采用双脱氧链末端终止法检测ErbB2基因rs1058808位点多态性,并分析两组及不同民族基因型和等位基因的分布情况.结果 病例组ErbB2基因rs1058808位点的CC、CG和GG基因型所占比例分别为15.2％、46.9％、37.9％,对照组分别为14.7％、62.7％、22.7％,观察组GG基因型所占比例高于对照组(P＜0.01).病例组朝鲜族和汉族人群ErbB2基因rs1058808位点GG基因型所占比例分别为35.7％、36.8％,均高于对照的23.5％、22.0％(P均＜0.01);两组同民族CC、CG基因型及C、G等位基因分布差异无统计学意义,病例组汉族和朝鲜族人群CC、CG和GG基因型及C、G等位基因分布差异无统计学意义(P均＞0.05).结论 ErbB2基因rs1058808位点GG基因型可能是延边地区人群HBV相关HCC发生的主要遗传易感因素,且汉族和朝鲜族间不存在民族差异.%Objective To investigate the rs1058808 polymorphism of ErbB2 gene in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) in Yanbian area,and to explore the correlation between rs1058808 polymorphism and HBV-related HCC and ethnic differences.Methods The gene polymorphisms of rs1058808 in ErbB2 of 66 cases of patients with HBV associated HCC (the experimental group,including 28 cases of Korean nationality and 38 cases of Han nationality) and 150 cases of pure HBV carriers (the control group,including 68 cases of Korean nationality and 82 cases of Han nationality) were detected by dideoxy chain-termination method.We analyzed the genotype and allele distribution in the two groups.Results Both the two groups had rs1058808 ErbB2 gene loci of CC,CG and GG genotypes,and G gene was the most allele.The proportions of CC,CG and GG genotypes of ErbB2 gene rs1058808 loci in the experimental group were 15.2％,46.9％ and 15.2％,while in the control group they were 14.7％,62.7％ and 22.7％;the proportion of GG genotype in the experimental group was higher than that of the control group (P ＜0.01).The proportions of rs1058808 ErbB2 gene loci GG genotype in the Korean nationality and Han nationality of the experimental group were 35.7％,36.8％,which were higher than those of the control group (23.5％,22.0％)(all P ＜0.01).There was no statistically significant difference between the two national groups in CC,CG genotypes,C and G allele distribution.In the experimental group,there was no statistically significant difference in CC,CG and GG genotypes and C and G allele distribution between Han and Korean patients (all P＞0.05).Conclusion The GG genotype of rs1058808 in ErbB2 may be the main genetic risk factor for HBV-associated HCC in Yanbian area,and there is no national differences between Han and Chinese-Korean patients.