阿胶对气道炎症小鼠Th17/Treg亚群失衡的逆转作用

摘要

目的 探讨阿胶对烟尘所致气道炎症小鼠肺脏Th17/Treg亚群失衡的逆转作用.方法 24只C57BL/6小鼠,随机分为对照组、模型组和阿胶组.模型组、阿胶组利用香烟烟雾暴露法建立气道炎症模型,模型组小鼠每日0.2 mL PBS灌胃,阿胶组每日给予0.2 mL阿胶溶液(0.2 g/mL)灌胃,正常组常规饲养.24周后,观察各组小鼠肺组织病理情况,采用流式细胞术检测肺组织Th17细胞亚群及Treg细胞亚群比例,采用逆转录PCR法检测肺组织中IL-6、IL-17A、Foxp3 mRNA表达,采用酶联免疫吸附法检测血清IL-6、IL-17A、Foxp3水平.结果 与正常组相比,模型组小鼠肺间质有大量炎性细胞浸润,肺泡破裂融合,肺组织Th17细胞、Treg细胞比例高,血清IL-17A、IL-6、Foxp3水平和肺组织IL-17A、Foxp3、IL-6 mRNA表达高(P均＜0.05).与模型组相比,阿胶组小鼠肺组织炎症情况明显改善,肺组织Th17细胞、Treg细胞比例低,血清IL-17A、IL-6、Foxp3水平和肺组织IL-17A、Foxp3、IL-6 mRNA表达低(P均＜0.05).结论 烟尘能导致气道炎症小鼠肺组织Th17、Treg亚群比例升高,IL-6、IL-17A、Foxp3细胞因子表达上调,阿胶通过降低Th17、Treg亚群比例及IL-6、IL-17A、Foxp3细胞因子表达减轻气道炎症小鼠肺脏炎症.%Objective To investigate the therapeutic effect of Ejiao (colla corii asini) on the imbalance of Th1T/Treg subsets in the lung tissues of mice with smoking-induced airway inflammation.Methods Twenty-four male C57BL/6 mice were randomly divided into the control group,model group,and Ejiao (colla corii asini) group (Ejiao group).Mice in the model group and Ejiao group were exposed to cigarette smoking.Mice in the model groupwere administered 0.2 mL PBS daily,mice in the Ejiao group were administered 0.2 mL Ejiao (colla corii asini) solution (0.2g/ml) daily,and mice in the control group were fed normally.Twenty-four weeks later,the histopathological condition of lung in mice of each group was observed.The proportion of helper T cell 17 subsets (Th17) and regulatory T cell subsets (Treg) in the lung tissues were detected by flow cytometry.The expression of IL-6,IL-17A,and Foxp3 mRNA in the lung tissues was detected by reverse transcription polymerase chain reaction (RT-PCR),and the serum levels of IL-6,IL-17A,and Foxp3 were detected by enzyme-linked immunosorbent assay.Results Compared with the control group,the lung tissues of mice in the model group were infiltrated with large number of inflammatory cells,alveoli ruptured and fused,the proportion of Th17 cells and Treg cells in the lung tissue increased,and the levels of IL-17A,IL-6,Foxp3 in the serum,and IL-17A,Foxp3,and IL-6 mRNA in the lung tissues increased (all P ＜ 0.05).Compared with the model group,the inflammation of the lung tissues of mice in the Ejiao group was significantly improved,the proportion of Th17 cells and Treg cells in the lung tissues decreased,and the levels of serum IL-17A,IL-6,and Foxp3,and IL-17A,Foxp3,and IL-mRNA in the lung tissues decreased (all P ＜ 0.05).Conclusions Smoke can induce the increased proportion of Th17 and Treg subsets and up-regulate the expression of IL-6,IL-17A,and Foxp3 cytokines in the lung tissues of mice with airway inflammation.Ejiao (colla corii asini) can reduce the lung inflammation in mice by reducing the proportion of Thl7 and Treg subsets and expression of IL-6 and IL-17A,and Foxp3.