发根农杆菌菌株和花生品种对发根诱导率的影响

摘要

试验以花育20、花育45、鲁花11无菌花生子叶为材料,采用3×3双因子设计,研究3株发根农杆菌菌株和花生品种对花生发根诱导率的影响.结果表明:发根农杆菌和花生品种均显著影响发根诱导率.花育45的发根诱导率最高,为93.7%,其适宜菌株为 A4;花育20的发根诱导率为89.2%,适宜菌株为ATCC15834;鲁花11的发根诱导率为73.4%,适宜菌株为GIM1.141.%Three Agrobacterium rhizogenes strains on the induction rate of hairy roots of peanut were in-vestigated by 3×3 double-factor design with the cotyledon of sterile plantlets of Huayu 20,Huayu 45,Luhua 11 as meterials.The results showed that both Agrobacterium rhizogenes and peanut varieties significantly affect-ed the induction rate of hairy roots.Huayu 45 had the highest induction rate of 93.7%, and its appropriate strain was A4.The hairy roots induction rate of Huayu 20 was 89.2%,and the suitable strain was ATCC15834. The hairy roots induction rate of Luhua 11 was 73.4%,and its fitting strain was GIM1.141.