伏核微量注射异丁司特对吗啡成瘾大鼠甩尾痛阈的影响

摘要

Objective The aim of this study was to investigate the effect of microinjection ibudilast on the appendix pain threshold in nucleus accumbens(NAC)of morphine-addicted rats. Methods Normal rats and morphine-addicted rats were injec-ted with ibudilast(5 μg/2 μL)or saline(2 μL)into NAC through glass microelectrode. The tail-flick period(TFL)was used as an in-dicator to observe the thermal pain threshold before and after ibuilast injection. Results After injected ibudilast 5 μg into the NAC of normal rats and morphine-added rats,the TFL was prolonged compared with that before injection,and the TFL prolongation of mor-phine-addicted rats was more significant than that of normal rats. There was no significant change in TFL after 2 μL of saline was in-jected into the NAC of normal rats and morphine-addicted rats. Conclusion Microinjection of ibudilast into NAC of normal rats and morphine-added rats can cause analgesic effect,and the analgesic effect of morphine-addicted rats is more significant.%目的 研究伏核(Nucleus accumbens NAC)内微量注入异丁司特对吗啡成瘾大鼠甩尾痛阈的影响.方法 正常大鼠及吗啡成瘾大鼠伏核经玻璃微电极注入异丁司特(5 μg/2 μL)或生理盐水(2 μL)后,以甩尾潜伏期(TFL)为指标,观察注药前后的镇痛作用.结果 正常大鼠及吗啡成瘾大鼠伏核内注入5 μg的异丁司特后,与注药前相比,其TFL延长,并且吗啡成瘾大鼠TFL延长较正常大鼠更为显著;Wistar正常大鼠及吗啡成瘾大鼠伏核注入2 μL生理盐水后,TFL无显著变化.结论 大鼠及吗啡成瘾大鼠伏核内微量注入异丁司特后,可使其产生镇痛作用,并且吗啡成瘾大鼠的镇痛效果更为显著.