葫芦种子传黄瓜绿斑驳花叶病毒的检测

摘要

从感染黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的葫芦植株上收取种子,通过苗期症状观察法、双抗体夹心酶联免疫吸附法(DAS-ELISA)、免疫捕获反转录PCR(IC-RT-PCR)法测定葫芦种子的带毒情况,并用生物学接种方法测定葫芦种千携带病毒的侵染活性.苗期症状观察法结果表明,199株幼苗有2株表现花叶斑驳症状,种子传毒率为1.01%;而利用DAS-ELISA和IC-RT-PCR法随机检测30粒葫芦病株种子,CGMMV检出率为100%.种子各部位携带的CGMMV接种葫芦表现典型的花叶斑驳症状,表明葫芦种子携带的CGMMV具有侵染活性.DAS-ELISA检测葫芦种子CGMMV的灵敏度为1/5120种子研磨液.%The seed transmission rate of Cucumber green mottle mosaic virus (CGMMV) in the bottle gourd Lagenaria siceraria was detected based on seedling symptoms, DAS-ELISA and immunocapture RT-PCR (IC-RTPCR). Seed transmission ratio of CGMMV in bottle gourd from the virus-contaminated seeds was 1.01％, while seed-borne ratio of CGMMV in bottle gourd seeds tested was 100％ by DAS-ELISA and IC-RT-PCR. CGMMV in bottle gourd seeds was demonstrated to have infection activity by biological inoculation. The sensitivity of DASELISA used for detection of CGMMV in the seed sap of bottle gourd was 1/5120.