Specific Primers for Detection of Cucumber green mottle mosaic virus

摘要

PURPOSE: Specific primers for detecting cucumber green mottle mosaic virus are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of cucumber green mottle mosaic virus, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting cucumber green mottle mosaic virus comprise a nucleic acid fragment selected from the nucleotide sequences set forth in SEQ ID NO: 2 to SEQ ID NO: 4 which species specifically bind with cucumber green mottle mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequences set forth in SEQ ID NO: 7 to SEQ ID NO: 9 which are combined with the selected nucleic acid and species specifically bind with cucumber green mottle mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.