滇重楼的内生真菌及真菌中甾体化合物的分析

摘要

Fifty endophytic fungal isolates were separated and purified from the rhizomes and seeds of Paris polyphylla var. yunnanensis collected in Yunnan Province of China. More endophytic fungi were separated from the phloem than from the seed or xylem. Detection and analysis methods for steroids were established. The steroids were detected in the fermentation cultures of thirty-two fungal isolates. It was found that five fungal isolates could produce steroids with a yield exceeded 50 mg/L.%从采自云南的滇重楼(Paris polyphylla var.yunnanensis)根状茎韧皮部、木质部和种子中分离出50株内生真菌,其中从根状茎的韧皮部中分离出内生真菌最多.建立了甾体类化合物的检测和分析方法.检测到32株内生真菌的发酵培养物中含有甾体化合物,其中5株内生真菌的发酵培养物中甾体化合物的产率超过了50 mg/L.