海洋来源放线菌3275化学成分及抗肿瘤活性研究

摘要

In order to find bioactive compounds, the secondary metabolites of the fermentation of actinomycete streptomyces sp.3275 were studied,Among seven compounds were isolated from the active extract by repeated chromatography over silica gel,sephadex LH-20 column and HPLC. Their structures were identified on the basic of comparison to literature and spectroscopic analysis as cyclo (Val-Pro) (1), cylco (Pro-Tyr) (2), cyclo (Gly-Pro) (3), cyclo (Pro-Ile) (4), cyclo (Leu-Pro) (5),cholest-5-en-3β-ol (6), 1-methyl- 1,2,3,4-tetrahydro-carboline-3-carboxylic acid (7). Compounds 2,3,5 exhibited weak cytotoxicities against tsFT210 cell line.%从海洋来源的放线菌.Streptomyces sp.3275中分到7个化合物,经IR,FSI-MS,NMR等光谱数据鉴定及与文献对照,确定其结构为环(脯-缬)(1),环(脯-酪)(2),环(脯-甘)(3),环(脯-异亮)(4),环(脯-亮)(5),胆甾醇(6)及1-甲基-1,2,3,4-四氢咔啉-3-羧酸(7);并采用SRB法对其抗肿瘤活性进行了测试,其中化合物2,3,5对温敏型小鼠乳腺癌细胞tsFT210显示弱的增殖抑制活性.