粗根荨麻水提取部分对佐剂性关节炎大鼠腹腔巨噬细胞分泌TNF-α及PGE2的影响

摘要

To investigate the effects of aqueous fraction of Urtica macrorrhiza Hand-Mazz(Ur) on modulating tumor nec- rosis factor-alpha (TNF-α)and prostaglandin E2 (PGE2) production induced by lipopolysaceharide (LPS) in peritoneal macrophages in adjuvant arthritis rats and elucidate the possible mechanisms of anti-inflammatory and antirheumatoid effects of Ur, adjuvant arthritis (AA) rat was used as the model. The PMψ samples were taken at different time after medication. TNF-α, PGE2 levels were :measured by ELISA method. Production of TNF-α, and PGE2 increased in the cul-ture supematant of PMψ in AA model rats. Ur(400 and 200 mg/kg) could inhibit TNF-α and PGE2 release induced by LPS from PMψ in AA rats. The anti-inflaramatory mechanisms of Ur in AA rats might be reIated to its inhibitory effects on the level of TNF-α and PGE2 from PMψ in vivo.%观察滇产粗根荨麻水提取部分对佐剂性关节炎(adjuvant arthritis,AA)大鼠腹腔巨噬细胞(peritoneal macrophages,PMcp)分泌肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)及前列腺素E2(prostaglandin E2,PGE2)的影响.建立大鼠佐剂性关节炎模型,Ur水提取部分连续灌胃给药14或21 d后分次获取大鼠腹腔巨噬细胞,脂多糖(lipopolysacehafide,LPS)诱导大鼠腹腔巨噬细胞,用酶联免疫吸附法检测培养上清液中TNF-α及PGE2水平.从大鼠腹腔巨噬细胞TNF-α及PGE2分泌较正常组升高,Ur水提取部分(400,200 mg/kg)对LPS诱导的AA大鼠腹腔巨噬细胞分泌TNF-α及PGE2水平有明显抑制作用.滇产粗根荨麻水提取部分对佐剂性关节炎的治疗作用可能与其抑制腹腔巨噬细胞分泌TNF-α及PGE2有关.