牡蒿提取物抗氧化作用和遗传毒性研究

摘要

本文旨在研究牡蒿提取物(Artemisiajaponica Extract,AJE)对小鼠抗氧化作用和遗传毒性效果.在建立卡介苗(BCG)+脂多糖(LPS)诱导免疫性肝损伤小鼠模型的基础上,采用给小鼠灌胃不同浓度的AJE后,分别测定小鼠肝组织中丙二醛(MDA)、谷胱甘肽氧化酶(GSH-Px)和超氧化歧化酶(SOD)的活性.检查小鼠骨髓嗜多染红细胞微核率和小鼠精子畸形率.结果表明,在一定剂量范围内,牡蒿提取物有降低小鼠肝组织中MDA含量、升高GSH-Px和SOD活性的作用,微核试验和精子畸形试验结果均为阴性.说明AJE具有较强的抗氧化作用,无遗传毒性.%The experiment was conducted to determine the effects of Artemisia japonica Extract (AJE) on antioxidant parameters and genetic toxicity in mice. Based on the model of immunological liver injury in mice established by injection of Bacillus Calmette Guerin (BCC) and lipopolysaccharide (LPS) from tail vein, mice were fed with AJE of various concentrations. The activities of MDA,GSH-Px,and SOD in mice livers were measured and the results of micronucleus and sperm abnormality were examined. The results showed that the content of MDA in livers was decreased while the activities of GSH-Px and SOD was increased by AJE within certain range of dosage. However,the results of micronucleus and sperm abnormality tests were negative. It was proved that AJE possessed of the relatively high anti-oxidant function and no genetic toxicity effect.