To investigate the effect of psoralen on the proliferation and correlated factors of hepatic stellate cells ( HSCT6) in vitro. Psoralen was dissolved in dimethyl sulphoxide (DMSO) at different concentrations of 10 μmmoL/L, 1 μmmol/L,0. 1 μmmol/L, and 0. 01 μmmol/L. The cell proliferation was assessed with methyl thiazolyl tetrazolium (MTT) after incubation with psoralen for 24 h,48 h,and 72 h,respectively. HSC-T6 were divided into 5 groups and incubated in the presence of 0.1 mol/L hydrogen dioxide followed by washing with PBS for 3 times. Psoralen at different concentrations (10 μmol/L, 1 . μnol/L,0. 1 μmol/L) was added into the cell culture media,and after incubatio for 48 h,the activities or levels of superoxide dismutase (SOD),malonaldehyde (MDA) ,glutathione (GSH),glutathione peroxidase (GSH-Px),and collagen I (COL I) in the supematant of the cell culture were measured. The results showed that psoralen inhibited the cell proliferation at the concentration of 10, 1, and O. 1 μnol/L. Psoralen significantly decreased the production of extracellular MDA, GSH, and COL I and increased SOD and GSH-Px activity. Therefore, psoralen can inhibit the proliferation of HSC-T6,decrease lipid peroxiclation in HSC-T6, and degrade the secretion from COL I in HSC-T6.%通过研究植物雌激素香豆素补骨脂素对体外培养的大鼠肝星状细胞HSC-T6增殖及相关因子表达的影响,为补骨脂素治疗肝纤维化提供实验依据.常规培养肝星状细胞HSC-T6,采用0.1 mmol/L的H202制造HSC-T6氧化应激的模型.分别用MTT法检测肝星状细胞增殖、放射免疫法检测细胞上清液中超氧化物歧化酶(SOD),丙二醛(MDA),还原性谷胱甘肽(GSH),谷胱甘肽过氧化物酶(GSH-Px)的活性和含量,ELISA法测定Ⅰ型胶原的分泌.结果表明:与正常对照组组比较,补骨脂素在浓度为10 μmol/L,1 μmol/L,0.1 μmol/L,均呈现出抑制HSC-T6增殖的作用(P＜0.05),且最佳作用时间为48 h(P＜0.05);与模型组比较,补骨脂素各个浓度组能够提高SOD和GSH-Px的活性(P＜0.05),并降低细胞上清液中MDA和GSH的含量(P＜0.05);与模型组比较,补骨脂素各个浓度组在作用48 h后,细胞上清液中的Ⅰ型胶原的表达量均降低(P＜0.05).因此,作为植物雌激素的一种,补骨脂素能有效的抑制HSC-T6的增殖及抗HSC-T6氧化应激,很可能成为雌激素的替代品在治疗肝纤维化中.
展开▼
