双水相体系萃取人参根中人参皂苷的研究

摘要

建立稳定的聚乙二醇(PEG)与(NH4)2 SO4双水相体系以分离人参根中人参皂苷.通过上下相体积比(R)、分配系数(K)和回收率(Y)分析双水相体系对人参皂苷的萃取效果,研究了PEG分子量、PEG/(NH4)2SO4质量分数、pH值和温度等因素对双水相成相及人参皂苷萃取的影响.结果表明:PEG分子量为3350、PEG3350的质量分数为12％、(NH4)2SO4质量分数为16％、溶液pH为7.0、温度为60℃时,双水相体系对人参皂苷有较高的萃取率,回收率可到达88.94％.%The ginsenosides were extracted and purified by PEG/(NH4)2SO4 aqueous two-phase system (ATPS) from the roots of Panax ginseng C. A. Meyer. The extraction parameters of ATPS,including the molecular weight of PEG,concentration of PEG/(NH4)2SO4 ,pH and temperature were investigated based on volume ratio (R) ,partition coef cient (K) and recovery yield (Y). The results indicated that the optimal ATPS was 12% PEG3350 and 16% (NH4)2SO4at pH 7.0 and 60 ℃. The recovery yield of ginsenosides using the optimized ATPS was 88.94%.