柠条锦鸡儿籽油体外抗真菌作用及其机制研究

摘要

参照美国临床实验室标准化委员会(NCCLS)推荐的《产孢丝状真菌的液基稀释法抗真菌药物敏感性试验方案》(M38-A),测定超临界CO2萃取的柠条锦鸡儿籽油对3种(每种15株)临床常见的皮肤癣菌的最低抑菌浓度(MIC)；高效液相色谱法研究柠条锦鸡儿籽油对真菌细胞膜麦角甾醇生物合成的影响以探讨其作用机制.结果表明柠条锦鸡儿籽油对犬小孢子菌、须癣毛癣菌、红色毛癣菌的MIC范围分别为64～512 μg/mL、32～512μg/mL、64～1024 μg/mL;高效液相色谱法检测显示,与其生长对照菌相比,籽油作用的皮肤癣菌细胞膜上麦角甾醇含量明显降低,且具有剂量依赖性.柠条锦鸡儿籽油主要是通过影响真菌细胞膜上麦角甾醇的合成而发挥抗真菌作用.%The minimal inhibitory concentration (MIC) of Caragana korshinskii Kom.seed oil against 3 kinds (each kind 15 strains) of common clinical dermatophytes were determined in vitro using the standard protocol approved by the National Committee for Clinical Laboratory Standards (NCCLS).The seed oil from C.korshinskii was extracted by supercritical CO2 fluid.The MIC range of seed oil against Microsporum canis,Trichophyton mentagrophytes,Trichophyton rubrum was 64-512 μg/mL,32-512 μg/mL and 64-1024 μg/mL,respectively.In addition,the possible inhibitory mechanism of C.korshinskii seed oil on the biosynthesis of ergosterol in cell membrane was investigated by high performance liquid chromatography (HPLC) analysis.The HPLC analysis results showed that C.korshinskii seed oil inhibited the ergosterol biosynthesis in cell membrane of dermatophytes in a dose dependent manner.Hence,it was deduced that the seed oil mainly influenced the ergosterol biosynthesis to achieve the anti-fungal activity.