In this study,a comprehensive UPLC-PDA-MS method was developed,optimized and validated to investigate the chemical components of Coptis-Scute composite herbal pair. Chromatographic separation was carried out using an Ac-quity UPLC BEH C18 column eluted with a mobile phase consisting of 0.05% formic acid in water and methanol. Twelve main peaks were detected in the chromatogram of Coptis-Scute sample based on the comparison of retention times, on line UV spectra and MS spectra of the standards and different samples. Eight components were identified and confirmed,two components were tentatively assigned. In addition,the concentrations of the identified eight components were quantified. The relationship between the concentration and the peak areas of the eight compounds was all linear. The RSDs of precision and reproducibility were all less than 5.0%. The average recoveries was 95% to 105%. The method developed in this study was rapid, simple, reliable and accurate. It had been successfully used for the quantification of eight compounds in Coptis-Scute composite herbal pair.%本文建立了黄连-黄芩药对化学成分的UPLC-PDA-MS分析方法.实验采用ACQUITY UPLC BEH C18 柱,流动相为0.05％甲酸水和甲醇梯度洗脱；检测波长280 nm;MS一级全扫描模式.综合分析标准品及不同样品的色谱峰保留时间、紫外光谱及MS一级全扫描质谱图,归属了黄连-黄芩提取液中的12个主要色谱峰,鉴别出8种成分,推断出2种成分,同时对鉴别出的8种成分进行了定量,被测成分在线性范围内均具有良好的线性关系(r≥0.9991),精密度、重复性的RSD均小于5.0％,加样回收率基本在95％～105％内.本方法快捷、准确,重复性好,能同时测定8个主要化学成分,可较全面地控制黄连-黄芩药对化学成分的含量.
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