归芪多糖延缓衰老作用的研究

摘要

In order to observe the anti-aging effect of Guiqi polysaccharide on aging mice induced by D-gal. We determined the activity of hydrogen peroxide (H2O2) in serum,monoamine oxidase (MAO) in brain,hydroxyproline (Hyp) in skin, glutathione peroxidase (GSH-PX) and xanthine oxidase (XOD) in liver tissue with senile model caused by D-gal (0. 5 g · kg-1 Vd) and meanwhile administered with Guiqi polysaccharide of 1 g · kg-1/d and 0. 5 g · kg-1/d o-rally. It was found that Guiqi polysaccharide can obviously increase the activation of GSH-PX, Hyp, and the XOD in liver tissue, and decrease the level of H2 O2 , XOD and MAO of aging mice. The results proved that Guiqi polysaccharide had the obvious effect of antisenility.%本文研究了归芪多糖对衰老小鼠的抗衰老作用.将50只小鼠分为5组,除正常对照组外其余各组均采用D-半乳糖(0.5 g·kg-1)皮下注射建立衰老小鼠模型,以不同剂量的归芪多糖(1 g/kg和0.5 g/kg)给小鼠连续灌胃42 d后,测定其血清中过氧化氢(H2O2)和皮肤组织中羟脯氨酸(Hyp)含量、大脑中单胺氧化酶(MAO)、肝组织中谷胱甘肽-过氧化物酶(GSH-PX)和黄嘌呤氧化酶(XOD)的活性.结果显示,归芪多糖能明显抑制小鼠血清H2O2含量、肝组织XOD和大脑MAO的活性(P＜0.01)；显著提高肝组织中GSH-PX的活性和皮肤组织中Hyp的含量(P＜0.01).证明归芪多糖可能通过降低衰老小鼠血清H2O2的含量、肝组织XOD和大脑MAO的活性,以及提高肝组织GSH-PX的活性和皮肤组织中Hyp的含量的机制起到一定的抗衰老作用.