壳寡糖对环江香猪生化参数及肠道菌群组成的影响

摘要

为了探讨壳寡糖(COS)用作环江香猪饲料添加剂的可行性,试验选用21日龄断奶的环江香猪12头,随机分为2组,每组6头,分别饲喂添加0.5％ COS的基础日粮和添加抗生素的基础日粮,试验期为14 d.试验结束时采集仔猪血液,肝素抗凝,离心分离血浆,测定生化参数；处死后取回肠和盲肠内容物,用荧光定量PCR测定其中双歧杆菌、乳酸杆菌、链球菌、大肠杆菌和总细菌的数量.结果表明,与对照组相比,壳寡糖组仔猪碱性磷酸酶活性显著增加(P＜0.05),血氨和尿素氮含量有降低趋势(P＞0.05)；壳寡糖组回肠乳酸杆菌数量显著增加、链球菌数量显著降低(P＜0.05),盲肠双歧杆菌和乳酸杆菌数量显著增加、大肠杆菌和链球菌数量显著降低(P＜0.05).上述结果提示,壳寡糖可在一定程度上改善环江香猪蛋白质代谢和肠道菌群平衡,从而增强其肠道健康.%This study was conducted to investigate the feasibility of chitooligosaccharide (COS) as feed additive in Huanjiang mini-piglets. Twelve Huanjiang mini-piglets weaned at 21 days of age were randomly allocated into two groups with six replicates. The piglets were fed with an antibiotic- or 0. 5% COS-supplemented diet for 14 days. At the end of this trail, the blood of the piglets was collected. The plasma were separated and used for measuring biochemical parameters , and the contents from the ileum and cecum were collected for estimating the levels of Bifidobacterium, Lactobacillvs, Peptostreptococcus ,Escherichia and total bacteria by real-time quantitative PCR. The results showed that dietary supplementing COS increased (P < 0.05 ) the plasma activity of alkaline phosphatase, while decreased (P > 0.05 ) the plasma contents of urea nitrogen and ammonia,when compared with the control group. The PCR analysis showed that the COS enriched (P<0.05) the amounts of Lactobacillus in ileum and Bifidobacterium and Lactobacillus in cecum,while suppressed ( P < 0.05 ) the amounts of Peptostreptococcus in ileum and Peptostreptococcus and Escherichia in cecum. Collectively, these findings suggested that the COS may improve protein metabolism marginally, and enhance intestinal health by regulating the intestinal flora in Huanjiang mini-piglets.