石油菜挥发油和超临界流体萃取物化学成分的GC-MS分析

摘要

首次利用水蒸气蒸馏法和超临界流体萃取法(SFE)对石油菜地上部位进行提取,分别获得挥发油和SFE萃取物,采用气相色谱-质谱联用技术(GC-MS)结合计算机数据库检索对这两种提取物的化学成分进行了分析鉴定,采用峰面积归一化法测定了其中各个化学成分的相对含量.从挥发油中共分离出39个色谱峰,鉴定了其中28个化学成分,已鉴定出的成分的相对含量总和占化合物检出总量的91.90％,化合物类型均为萜类.从SFE萃取物中共分离出136个色谱峰,鉴定了其中65个化学成分,已鉴定出的成分的相对含量总和占化合物检出总量的58.17％,化合物类型包括萜类、芳香族类和脂肪族类.挥发油和SFE萃取物均含有α-蒎烯、石竹烯和桉油烯醇,其他成分则存在较大差异.%The aerial part of Pilea cavaleriei Levl.was extracted by steam distillation and supercritical fluid extraction (SFE)methods for the first time.The volatiles and the SFE extracts were then analyzed by GC-MS and matched with commercial libraries.The relative content of each component was determined by area normalization.39 peaks were separated from the volatiles and 28 of them were identified,representing about 91.90％ of the total content.All of the identified components of the volatiles were terpenoids.136 peaks were separated from the SFE extracts and 65 of them were identified,representing about 58.17％ of the total content.The types of the components of the SFE extracts contained terpenoids,aromatics and aliphatics.α-Pinene,caryophyllene and spathulenol were both included in the volatiles and the SFE extracts,while the other chemical components were different.