HPLC-MS/MS法测定黄山贡菊花、叶和茎中酚类物质

摘要

The phenohc components of flower,leave and stem in Huangshan tribute chrysanthemum were analyzed by HPLC-MS/MS.The results showed that flavonoids and phenolic acids were the major phenolic components.Thirteen flavonoids and nine phenolic acids were detected in the flower of C.morifolium,eleven flavonoids and six phenolic acids were detected in the leaf of C.morifolium,and eight flavonoids and five phenolic acids were detected in the stem of C.morifolium.Meanwhile,components including chlorogenic acid,1,5-di-caffeoylquinic acid,3,5-di-caffeoylquinic acid,4,5-di-caffeoylquinic acid,apigenin-6-C-xylosyl-8-C-glucos-yl,apigenin-6-C-glucosyl-8-C-arabinosyl,apigenin-6-C-arabinosyl-8-C-glucosyl,luteolin-7-O-glucoside,luteolin-7-O-glucuronide,diosmetin-7-O-6"-malonylglucoside,andacacetin-7-O-6"-malonylglucoside were also detected with different concentrations in flower,leaf and stem of C.morifolium.However,acacetin-7-O-galactoside was detected in both leaf and stem of C.morifolium but was not detected in flower of C.morifolium,and apigenin-7-O-glucuronide was only detected in the leaf of C.morifolium.%采用HPLC-MS/MS检测技术分析了黄山贡菊花、叶、茎中酚类物质.结果表明,黄山贡菊花、叶、茎中酚类物质主要为黄酮及酚酸类化合物,其中贡菊花中含有13种黄酮类化合物,9种酚酸类化合物；叶中含有11种黄酮类化合物,6种酚酸类化合物；茎中含有8种黄酮类化合物,5种酚酸类化合物.贡菊花、叶、茎中共有的酚性成分包括:绿原酸、1,5-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸、芹菜素-6-C-木糖-8-C-葡萄糖、芹菜素-6-C-葡萄糖-8-C-阿拉伯糖、芹菜素-6-C-阿拉伯糖-8-C-葡萄糖、木犀草苷、木犀草素-7-O-葡萄糖醛酸苷、香叶木素-7-O-6”-丙二酰-葡萄糖苷、金合欢素-7-O-6”-丙二酰-葡萄糖苷.金合欢素-7-O-半乳糖苷存在于贡菊茎、叶中,而在贡菊花中未发现；芹菜素-7-O-葡萄糖醛酸苷只存在于贡菊叶中,在贡菊花和茎中均未发现.