观赏性珍稀濒危植物红皮糙果茶枝叶化学成分研究

摘要

在开展中国特有珍稀濒危植物化学成分及其生物功能研究中,我们运用硅胶、MCI和葡聚糖凝胶柱色谱以及半制备HPLC等分离纯化方法,首次对观赏性植物红皮糙果茶(Camellia crapnellia)枝叶中的化学成分进行了研究,得到7个单体化合物.结合理化性质和波谱数据与文献值对照分析鉴定了它们的结构,分别为:(-)-pinoresinol(1)、(±)-syringaresinol(2)、(±)-lariciresinol(3)、cytochalasin D (4)、blumenol B(5)、kaempferol-3-O-L-rahmnoside (6)和α-spinasterol(7).其中化合物2～5为首次从山茶属植物中分离得到.对分离所得化合物进行了蛋白酪氨酸磷酸酯酶1B(PTP1B)抑制活性测试,结果显示均无明显活性.%During our continuing research towards the discovery of bioactive natural products from rare and endangered plants endemic to China,the twigs and leaves of the ornamental plant Camellia crapnellianahave been initially,phytochemically investigated,which led to the isolation of seven miscellaneous compounds by repeated column chromatography (CC) over silica gel,MCI,Sephadex LH-20 and semi-preparative HPLC.By comparing their spectroscopic data and physicochemical properties with those reported in the literature,their structures were identified as (-)-pinoresinol (1),(±)-syringaresinol (2),(±)-lariciresinol (3),cytochalasin D (4),blumenol B (5),kaempferol-3-O-L-rahmnoside (6),and α-spinasterol (7),respectively.Among them,compounds 2-5 were reported from the genus Camellia for the first time.All the isolates were evaluated for their inhibitory effects against the protein tyrosine phosphatase 1B (PTP1 B),but none of them were active.