红花石蒜生物碱成分研究

摘要

On the basis of prophase studies in our laboratory,it was found that Lycorisradiata alkaloids had strong resistance activity against tobacco mosaic virus (TMV).In order to further study the mechanism of action,studyon the chemical compositions of L.radiate was carried out in this study.As a result,eleven compounds were isolated from L.radiate.Their structures were identified by 1H NMR,13C NMR,2D NMR and HR-ESI-MS as pancratinine D (1),hippeastrine (2),O-demethylhomolycorine (3),6o-O-methyllycorenine (4),lycoramine (5),tazettine (6),lycorine (7),lycoramine N-Oxide (8),O-demethylgalantamine (9),dihydrolycorine (10) and crinine (11).In addition,the anti-TMV activity of 1,3 and 10 were evaluated in vitro by inoculating half leaf method.All of them showed anti-TMV activity.The inhibition rate of 3 exceed positive control ningnanmycin.%我们实验室的前期研究发现红花石蒜中的石蒜西啶类生物碱具有较强的抗烟草花叶病毒(TMV)活性,为进一步研究其作用机制,开展了对红花石蒜化学成分的研究.从红花石蒜中共分离的到11个化合物,通过波谱数据结合理化性质鉴定(1H NMR、13C NMR、2D NMR、HR-ESI-MS)分别鉴定为pancratinine D(1)、小星蒜碱(2)、8-O-去甲基高石蒜碱(3)、6α-O-甲基石蒜伦碱(4)、力克拉敏(5)、水仙花碱(6)、石蒜碱(7)、力克拉敏N-氧化物(8)、去甲基加兰他敏(9)、二氢石蒜碱(10)、文殊兰碱(11).采用半叶枯斑法,对其中的三个化合物1、3、10进行了抗TMV活性筛选,发现均有一定的抗TMV活性.其中化合物3的抑制率超过阳性对照宁南霉素.