涨瓶强力枇杷露中有害微生物的筛选鉴定及其防治初步研究

摘要

采用平板涂布法从出现涨瓶现象的强力枇杷露样品中分离纯化有害菌,通过形态学、生理生化和分子生物学方法对其种属鉴定,且对该菌株的防治方法进行了初步研究.实验结果表明,从出现涨瓶现象的样品中筛选到一株有害菌LS2,种属鉴定结果表明,菌株LS2与拜耳接合酵母(Zygosaccharomyces bailii)特征最为接近,初步将其鉴定为拜耳接合酵母;防治方法研究结果表明,在110℃加热处理20 min时,可达到彻底杀灭要求.%In this study,spoilage microorganisms were isolated from bottle bulged Qiangli Pipa Syrup by spread plate method.The morphological,biochemical characteristics and molecular methods were analyzed to identify the taxonomic position of the strains.Moreover,their control methods were also studied in this test.The results showed that a spoilage strain(LS2) was obtained.According to taxonomic identification,the strain LS2 was identified as Zygosaccharomyces bailii.The sterilization requirements were achieved after heat treatment at 110 ℃ for 20 min.