恒山黄芪多糖对人宫颈癌细胞SiHa的抑制效应分析

摘要

本文旨在探讨道地药材恒山黄芪的多糖成分对人宫颈癌细胞SiHa生长和迁移能力的抑制效应.通过采用MTT法验证恒山黄芪多糖对SiHa细胞生长活力的抑制效应,利用细胞黏附试验和铺展试验检测恒山黄芪多糖对SiHa细胞的粘附能力和铺展能力的影响,并采用划痕试验检测恒山黄苠多糖对SiHa细胞迁移运动能力的抑制效应.结果表明:恒山黄芪多糖具有抑制SiHa细胞增殖的活性(IC50值为0.25 mg/mL),并且在低浓度(0.0625 mg/mL)时仍然能够有效抑制其生长;与对照细胞相比,恒山黄芪多糖处理的细胞在培养器皿底部的黏附能力和铺展能力显著下降(P＜0.01),并且明显抑制了SiHa细胞迁移运动能力(P＜0.01).因此,道地药材恒山黄芪的多糖成分具有抑制人宫颈癌细胞SiHa的增殖、粘附、铺展以及迁移运动的活性.%To evaluate the effects of Hengshan Astragalus polysaccharides on human cervical cancer cell SiHa,MTT assay was used to determine cell proliferation and viability of SiHa.The cell adhesive assay and cell spreading assay were used to detect the adhesive and spreading capabilities of SiHa,and the wound-healing assay was used to access the migration capability of SiHa.The results indicated that Hengshan Astragalus polysaccharides showed significant activity of suppressing SiHa cell proliferation (ICs0 value was 0.25 mg/mL) even at a low concentration (0.0625 mg/mL).Compared with the control group,the adhesive and spreading capability of SiHa cells treated with Hengshan Astragalus polysaccharides were obviously reduced (P ＜ 0.0l),and the migration capability was also depressed remarkably (P ＜ 0.01).Therefore,polysaccharides from the genuine medicinal material Hengshan Astragalus can inhibit the proliferation,adhesion,spreading and migration capability of human cervical cancer cell SiHa.