基于DNA条形码技术鉴别5种列当属药用植物

摘要

Objective:To establish a rapid,accurate and standardized method for identifying five species from genus Orobanche with DNA barcoding technology.Methods:Total DNA of 5 kinds of medicinal plants from genus Orobanche were extracted.ITS,rbcL,trnL-F and matK gene sequence amplified by PCR.PCR product was directly sequenced,and the results were aligned and analyzed with different software (CONTIG、BioEdit、MEGA6.0) to construct the phylogeny trees.Results:The 200 complete ITS,rbcL,trnL-F and matK sequences of 5 species from genus Orobanche have been obtained,and the length of 4 sequences region varied from 364 to 1240 bp.They were distinguished by trnL-F,ITS or matK sequence.Conclusion:RbcL sequence was not suitable to identify 5 species from the genus Orobanche,but ITS and trnL-F region could be a pretty good marker to distinguish these species,and it will be a potential molecular identification method for identifying Orobanche plants.%目的:建立一种快速、准确、标准化的5种列当属药用植物的DNA条形码鉴别方法.方法:采集5种列当属药用植物,所有样品进行总DNA提取,PCR扩增,并对扩增产物进行测序得到相应的序列,测序结果使用CONTIG、BioEdit、MEGA6.0软件进行分析,构建系统发育树.结果:测得5种列当属植物的ITS、rbcL、trnL-F和matK全长序列共200条,4种序列长度范围在364～1240 bp,其中ITS、trnL-F和matK可将其鉴别出来.结论:rbcL序列无法有效鉴别5种列当属药用植物,trnL-F与ITS序列能够成功鉴别,可作为列当属植物的分子鉴别方法.